Humaan MEMO1-antilichaam

MEMO1 (Mediator of ErbB2-driven cell motility 1) is a 297 amino acid protein. It is thought to relax extracellular chemotactic signals that are targeted at the microtubule cytoskeleton, thereby controlling cell migration. Additionally, MEMO1 is required for breast carcinoma migration, suggesting an important role in tumorigenesis. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity.

Protein MEMO1, C2orf4, CGI-27, MEMO, NS5ATP7

Human

Human

Recombinant human MEMO1 (1-297aa) purified from E. coli

AT1E9

Unconjugated

ELISA, WB, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

MEMO1, ATGA0566-10 µg, ATGA0566-20 µg, ATGA0566-50 µg, ATGA0566-100 µg, ATGA0566-250 µg, ATGA0566-500 µg, ATGA0566-1 mg, ATGA0566-10, ATGA0566-20, ATGA0566-50, ATGA0566-100, ATGA0566-250, ATGA0566-500, ATGA0566-1

Marone R., et al. (2004) Nat Cell Biol 6 (6) :515-522.; ; Meira M., et al. (2009) J Cell Sci 122:787-797.; ; Zaoui K., et al. (2010) Proc Natl Acad Sci USA 107 (43) :18517-18522.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_057039

Q9Y316

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MEMO1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Jurkat cell lysate; ; Lane 2.: U87MG cell lysate

Flow cytometry analysis of MEMO1 in U87MG cells. The cell was stained with ATGA0566 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .