Humaan MINCLE/CLEC4E antilichaam

CLEC4E (macrophage inducible C-type lectin, also called MINCLE or CLECSF9), which is a diverse family of protein which was originally defined by their ability to recognize a wide range of ligand of carbohydrate structure. CLEC4E expressed in macrophages subjected to several types of stress. It plays an essential role in response to trehalose-6, 6'-dimycolate (TDM) and activated by a synthetic analogue, trehalose dibehenate (TDB) . Recently it was reported that CLEC4E is associated with an immunoreceptor tyrosine-based activation motif-containing Fc receptor gamma chain (FcRgamma) and functions as an activating receptor for damaged self- and non-self-pathogenic fungi.

CLEC4E, CLECSF9, C-type lectin domain family 4 member E, C-type lectin superfamily member 9, Macrophage lectin 2, Macrophage-inducible C-type lectin, MINCLE, NtmntanCLEC4E Ege lectin 2

Human

Human

Recombinant human MINCLE (41-219aa) purified from E.coli

AT16E3

Unconjugated

ELISA, WB, ICC/IF, IHC, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

CLEC 4E, CLEC4E, CLECSF9, C-type lectin domain family 4, member E, C-type lectin domain family 4 member E, C-type lectin superfamily member 9, Macrophage lectin 2, Macrophage-inducible C-type lectin, MINCLE, NtmntanCLEC4E Ege lectin 2, ATGA0175-10 µg, ATGA0175-20 µg, ATGA0175-50 µg, ATGA0175-100 µg, ATGA0175-250 µg, ATGA0175-500 µg, ATGA0175-1 mg, ATGA0175-010, ATGA0175-020, ATGA0175-050, ATGA0175-100, ATGA0175-250, ATGA0175-500, ATGA0175-01M

Ishikawa E, et al. (2009) J Exp Med, 206 (13) :2879-88.; ; Matsunaga I, et al. (2009) J Exp Med, 206 (13) :2865-8.; ; Graham LM, et al. (2009) Cytokine, 48 (1-2) :148-55.

Seifert L, et al. The necrosome promotes pancreatic oncogenesis via CXCL1 and Mincle-induced immune suppression. (Nature. 2016) {https://pubmed.ncbi.nlm.nih.gov/27049944/}; ; Zhao X, et al. Activation of C-Type Lectin Receptor and (RIG) -I-Like Receptors Contributes to Proinflammatory Response in Middle East Respiratory Syndrome Coronavirus-Infected Macrophages. (J Infect Dis. 2020) {https://pubmed.ncbi.nlm.nih.gov/31562757/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Immunology

NP_055173

Q9ULY5

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CLEC4E antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : 293T cell lysate; ; Lane 2. : MINCLE transfected 293T cell lysate

Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human CLEC4E antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) .

Flow cytometry analysis of CLEC4E in LNCap cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

Immunofluorescence of human HeLa cells stained with monoclonal anti-human CLEC4E antibody (1:500) with Texas Red (Red) . Nucleus was stained by Hoechst 33342 (Blue) .