Humaan MINCLE/CLEC4E antilichaam

MINCLE (macrophage inducible C-type lectin, also called CLEC4E or CLECSF9), which is a diverse family of protein which was originally defined by their ability to recognize a wide range of ligand of carbohydrate structure. MINCLE expressed in macrophages subjected to several types of stress. It plays an essential role in response to trehalose-6, 6'-dimycolate (TDM) and activated by a synthetic analogue, trehalose dibehenate (TDB) . Recently it was reported that MINCLE is associated with an immunoreceptor tyrosine-based activation motif-containing Fc receptor gamma chain (FcRgamma) and functions as an activating receptor for damaged self- and non-self-pathogenic fungi.

CLEC4E, CLECSF9, C-type lectin domain family 4 member E, C-type lectin superfamily member 9, Macrophage lectin 2, Macrophage-inducible C-type lectin, MINCLE, NtmntanCLEC4E Ege lectin 2

Human

Human

Recombinant human CLEC4E (41-219aa) purified from E.coli

AT16E3

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

CLEC 4E, CLEC4E, CLECSF9, C-type lectin domain family 4, member E, C-type lectin domain family 4 member E, C-type lectin superfamily member 9, Macrophage lectin 2, Macrophage-inducible C-type lectin, MINCLE, NtmntanCLEC4E Ege lectin 2, ATGA0537-10 µg, ATGA0537-20 µg, ATGA0537-50 µg, ATGA0537-100 µg, ATGA0537-250 µg, ATGA0537-500 µg, ATGA0537-1 mg, ATGA0537-010, ATGA0537-020, ATGA0537-050, ATGA0537-100, ATGA0537-250, ATGA0537-500, ATGA0537-01M

Ishikawa E, et al. (2009) J Exp Med, 206 (13) :2879-88.; ; Matsunaga I, et al. (2009) J Exp Med, 206 (13) :2865-8.; ; Graham LM, et al. (2009) Cytokine, 48 (1-2) :148-55.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Immunology

NP_055173

Q9ULY5

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CLEC4E antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: CLEC4E Transfected 293T cell lysate;

Flow cytometry analysis of CLEC4E in LnCap cells. The cell was stained with ATGA0537 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

ICC/IF analysis of CLEC4E in HeLa cells. The cell was stained with ATGA0537 (1:500) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .