Humaan mNGF/proNGF Combo Snelle ELISA-test

Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released.

The Biosensis Human Mature NGF/proNGF Combo Rapid enzyme-linked immunosorbent assay (ELISA) Kit combines individual, but complementary ELISA kits for the two most important NGF isoforms: Mature NGF (BEK-2212) and full-length proNGF (BEK-2226) . Both kits are sandwich ELISAs, useful for the quantification of mature NGF and proNGF in less than 4 hours in cell culture supernatants, serum, and plasma (citrate), with a simplified protocol and no loss of sensitivity or specificity.

Beta-nerve growth factor; Ngfb; NGF; pro-beta nerve growth factor; proNGF

P01138

Human

See BEK-2212 & BEK-2226 for specific details

Mature NGF/proNGF Combo (BEK-2212/2226)

ELISA

Culture Supernatant, Plasma (Citrate), Serum

For research use only.

Shipped on ice packs

Store at 2-8°C

Mature NGF ELISA:proNGF ELISA:Cross-reactivity of NGF isoforms:Mature NGF ELISA:proNGF ELISA: Does not cross-react with recombinant human mature NGF and proBDNF tested at 25 ng/mL. Does not react with rodent proNGF. This NGF ELISA does not cross-react with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5, glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF165) tested at 25 ng/mL. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect mature NGF from numerous other species including mouse and rat.

Colorimetric

Human