Humaan Muscle Fosfofructokinase/PFKM antilichaam

PFKM (phosphofructokinase, muscle) is a regulatory glycolytic enzyme that convert fructose 6-phosphate and ATP into fructose 1, 6-bisphosphate (through PFK-1), fructose 2, 6-bisphosphate (through PFK-2) and ADP. Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease.

6-Phosphofructokinase muscle type, GSD7, PFK-1, PFK1, PFKA, PFKX, 6-Phosphofructokinase, muscle type 6 Phosphofructokinase Muscle Type, EC 2.7.1.11, MGC8699, PFKL, PFKM, PFKP, Phosphofructo 1 Kinase Isozyme A, Phosphofructokinase 1, Phosphofructokinase M, Phosphofructokinase, muscle, Phosphofructokinase, muscle type, Phosphofructokinase, polypeptide X, Phosphohexokinase

Human

Human

Recombinant human PFKM (1-780aa) purified from E. coli

AT2F11

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Muscle Phosphofructokinase, PFKM, ATGA0239-10 µg, ATGA0239-20 µg, ATGA0239-50 µg, ATGA0239-100 µg, ATGA0239-250 µg, ATGA0239-500 µg, ATGA0239-1 mg, ATGA0239-10, ATGA0239-20, ATGA0239-50, ATGA0239-100, ATGA0239-250, ATGA0239-500, ATGA0239-1

Martinez-Costa OH., et al. (2007) FEBS Lett. 581 (16) :3033-8. ; ; Kim JW., et al. (2004) Mol Cell Biol. 24 (13) :5923-36.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Metabolism

NP_000280

P08237

The HepG2 cell lysates (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PFKM antibody (1:500~2000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.