Humaan MyD88 antilichaam

Myeloid differentiation primary response gene 88 (MYD88) is adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. This protein increases IL-8 transcription. It is involved in IL-18-mediated signaling pathway. MYD88 activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes.

Myeloid differentiation primary response protein MyD88, MYD88D

Human

Human

Recombinant human MYD88 (1-309aa) purified from E. coli

AT22F11

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

MyD88, ATGA0247-10 µg, ATGA0247-20 µg, ATGA0247-50 µg, ATGA0247-100 µg, ATGA0247-250 µg, ATGA0247-500 µg, ATGA0247-1 mg, ATGA0247-10, ATGA0247-20, ATGA0247-50, ATGA0247-100, ATGA0247-250, ATGA0247-500, ATGA0247-1

Bonnert TP, et al. (January 1997) . FEBS Letters 402 (1) : 81-4.; ; Lord KA, et al. (1990) . Oncogene 5 (7) : 1095-7.; ; Arancibia SA, et al. (2007) . Biological research 40 (2) : 97-112.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_002459

Q99836

The cell lysate of Raji (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MYD88 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Flow cytometry analysis of MYD88 in A549 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of MYD88 in A549 cells. The cell was stained with ATGA0247 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .