Humaan MYL4 antilichaam

Myosin light chain 4 is a protein that in humans is encoded by the MYL4 gene. Myosin is a hexameric ATPase cellular motor protein. It is composed of two myosin heavy chains, two nonphosphorylatable myosin alkali light chains, and two phosphorylatable myosin regulatory light chains. This gene encodes a myosin alkali light chain that is found in embryonic muscle and adult atria. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.

Myosin light chain 4, Myosin, light chain 4, ALC1, AMLC, GT1

Human

Human

Recombinant human MYL4 (1-197aa) purified from E. coli

AT4E8

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

MYL4, ATGA0276-10 µg, ATGA0276-20 µg, ATGA0276-50 µg, ATGA0276-100 µg, ATGA0276-250 µg, ATGA0276-500 µg, ATGA0276-1 mg, ATGA0276-10, ATGA0276-20, ATGA0276-50, ATGA0276-100, ATGA0276-250, ATGA0276-500, ATGA0276-1

Kurabayashi, M., et al. (1998) J Biol Chem 263 (27) : 13930-6.; ; Rotter, M., et al. (1991) Nucleic Acids Res 19 (7) : 1497-504.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cytoskeleton

NP_001002841

P12829

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MYL4 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: HepG2 cell lysate; ; Lane 3.: K562 cell lysate; ; Lane 4.: MCF7 cell lysate; ; Lane 5.: Jurkat cell lysate; ; Lane 6.: 293T cell lysate; ; Lane 7.: LnCap cell lysate