Humaan N-Acetyl-D-Glucosamine Kinase/NAGK antilichaam

NAGK is a member of the eukaryotic-type N-acetylglucosamine kinase family. NAGK catalyzes the first committed step in arginine biosynthesis in organisms that perform the cyclic pathway of ornithine synthesis. In eukaryotic and bacterial oxygenic phototrophs, the activity of NAGK is controlled by the P (II) signal transduction protein. NAGK converts endogenous N-acetylglucosamine (GlcNAc), a key component of complex carbohydrates, from lysosomal degradation or alimentary sources into GlcNAc 6-phosphate.

N-acetyl-D-glucosamine kinase, GNK, HSA242910, GlcNAc kinase

Human

Human

Recombinant human NAGK (1-344aa) purified from E. coli

AT4D12

Unconjugated

ELISA, WB, ICC/IF

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

NAGK, ATGA0232-10 µg, ATGA0232-20 µg, ATGA0232-50 µg, ATGA0232-100 µg, ATGA0232-250 µg, ATGA0232-500 µg, ATGA0232-1 mg, ATGA0232-10, ATGA0232-20, ATGA0232-50, ATGA0232-100, ATGA0232-250, ATGA0232-500, ATGA0232-1

Weihofen WA, Berger M, Chen H, et al. (2007) . J. Mol. Biol. 364 (3) : 388-99.; ; Weidanz JA, Campbell P, Moore D, et al. (1997) . Br. J. Haematol. 95 (4) : 645-53.; ; Hinderlich S., et al. (Jul 2000) . Eur J Biochem 267 (11) : 3301-8.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Metabolism

NP_060037.2

Q9UJ70

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NAGK antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane a.: 293T cell lysate.; ; Lane b.: HeLa cell lysate.

ICC/IF analysis of NAGK in HeLa cells. The cell was stained with ATGA0232 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .