Humaan nanog antilichaam

Nanog is a multidomain homeobox transcription factor that functions to maintain the undifferentiated state of pluripotent stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. When Nanog expression is downregulated, cell differentiation can proceed. Proteins that regulate Nanog expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.

Nanog homeobox, hNanog, Homeobox protein NANOG, Homeobox transcription factor Nanog

Human

Human

Recombinant human Nanog (1-154aa) purified from E. coli

5A10

Unconjugated

ELISA, WB, IHC, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

Nanog homeobox, Nanog, Homeobox transcription factor Nanog-delta 48, Homeobox transcription factor Nanog, Homeobox protein NANOG, hNanog, FLJ40451, FLJ12581, , , ANA0837-10 µg, ANA0837-20 µg, ANA0837-50 µg, ANA0837-100 µg, ANA0837-250 µg, ANA0837-500 µg, ANA0837-1 mg, ANA0837-010, ANA0837-020, ANA0837-050, ANA0837-100, ANA0837-250, ANA0837-500, ANA0837-01M

Tay Y., et al. (2008) Nature. 455 (7216) :1124-8; ; Chiou SH., et al. (2008) Clin Cancer Res. 14 (13) :4085-95; ; Boer B., et al. (2008) Mol Reprod Dev.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Immunology

NP_079141

Q9H9S0

The cell lysate of NIH3T3 (35ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Nanog (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Paraffin embedded sections of human seminoma tissue were incubated with anti-human Nanog (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Flow cytometry analysis of Nanog in Hep3B cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.