Humaan NDRG1-antilichaam

N-myc downstream regulated gene (NDRG) -1 is one of 4 members of the NDRG alpha/beta-hydrolase family. This protein is classified in databases as a tumor suppressor and heavy metal-response protein. Its functions include cell-cycle regulation, cellular differentiation, apoptosis, hypoxia response and metal-ion sensing. Mutation in the NDRG1 gene has been reported to be causative for hereditary motor and sensory neuropathy-Lom (HMSNL), a severe autosomal recessive form of Charcot-Marie-Tooth (CMT) disease.

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Human

Human

Recombinant human NDRG1 (1-394aa) purified from E. coli

AT11G4

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

NDRG1, ATGA0401-10 µg, ATGA0401-20 µg, ATGA0401-50 µg, ATGA0401-100 µg, ATGA0401-250 µg, ATGA0401-500 µg, ATGA0401-1 mg, ATGA0401-10, ATGA0401-20, ATGA0401-50, ATGA0401-100, ATGA0401-250, ATGA0401-500, ATGA0401-1

Van Belzen N., et al. (1997) Lab Invest. 77 (1) : 85-92.; ; Kokame K., et al. (1997) J Biol Chem. 271 (47) : 29659-29665. ; ; Zhang J., et al. (2008) Gene. 417 (1-2) : 5-12.; ; Kachhap SK., et al. (2007) PLoS ONE. 2 (9) : e844.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Neuroscience

NP_006087

Q92597

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NDRG1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : LnCaP cell lysate ; ; Lane 2. : HeLa cell lysate ; ; Lane 3. : PC3 cell lysate ; ; Lane 4. : Hep3B cell lysate ; ; Lane 5. : HT1080 cell lysate ; ; Lane 6. : Jurkat cell lysate ; ; Lane 7. : SW480 cell lysate

ICC/IF analysis of NDRG1 in HeLa cells. The cell was stained with ATGA0401 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .