Humaan NKp44/NCR2-antilichaam

NKp44 (NCR2) is a member of the natural cytotoxicity receptor (NCR) family that is expressed on activated human NK cells. NKp44 displays a single extracellular Ig-like V domain and a transmembrane portion containing the charged residue (Lysine), likely involved in the association with KARAP/DAP12 molecules.

Natural cytotoxicity triggering receptor 2 isoform 1, CD336, LY95, NK-p44 NKP44, ANK0501

Human

Human

Recombinant human NKp44 (19-130aa) purified from E. coli

AT1G6

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

NKp44, NCR2, Natural cytotoxicity triggering receptor 2 isoform 1, CD336, LY95, NK-p44 NKP44, ATGA0322-10 µg, ATGA0322-20 µg, ATGA0322-50 µg, ATGA0322-100 µg, ATGA0322-250 µg, ATGA0322-500 µg, ATGA0322-1 mg, ATGA0322-10, ATGA0322-20, ATGA0322-50, ATGA0322-100, ATGA0322-250, ATGA0322-500, ATGA0322-1

Biassoni. R., et al., (2003) J Cell Mol Med 7: 376-387.; ; Kerry. S.C., et al., (2004) Journal of Immunology 172: 899-906.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Immunology

NP_004819

O95944

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NKp44/NCR2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human NKp30 protein; ; Lane 2.: Recombinant human NKp44 protein; ; Lane 3.: Recombinant human NKp46 protein;

Flow cytometry analysis of NKp44/NCR2 in Jurkat cells. The cell was stained with ATGA0322 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

ICC/IF analysis of NKp44/NCR2 in Jurkat cells. The cell was stained with ATGA0322 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .