Humaan NM23-H1/NME1-antilichaam

Non-metastatic cells 1 (NME1), also known as NM23-H1, originally identified as a candidate metastasis suppressor gene. NME1 is expressed in different tumor types where their levels have been alternatively associated with reduced or increased metastatic potential. Reductions in NME1 expression have been significantly associated with aggressive behavior in melanoma, breast, colon, and gastric carcinomas. On the contrary, high levels of NME1 gene expression are noted in the advanced stage of thyroid carcinomas.

NME/NM23 nucleoside diphosphate kinase 1, Nucleoside diphosphate kinase A, Granzyme A-activated Dnase, GAAD, Metastasis inhibition factor nm23, NM23-H1, Tumor metastatic process-associated protein, NDK A, NDP kinase A, NDPKA, NM23

Human

Human

Recombinant human NME1 (1-152aa) purified from E. coli

AT5F4

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

NM23-H1, ATGA0461-10 µg, ATGA0461-20 µg, ATGA0461-50 µg, ATGA0461-100 µg, ATGA0461-250 µg, ATGA0461-500 µg, ATGA0461-1 mg, ATGA0461-10, ATGA0461-20, ATGA0461-50, ATGA0461-100, ATGA0461-250, ATGA0461-500, ATGA0461-1

Tee YT., et al. (2006) Taiwan J Obstet Gynecol. 45 (2) : 107-13.; ; Negroni A., et al. (2000) Cell Death Differ. 7 (9) : 843-50.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Epigenomics (Transcription & Translation)

NP_000260

P15531

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1 : HeLa cell lysate; ; Lane 2 : A549 cell lysate; ; Lane 3 : Jurkat cell lysate; ; Lane 4 : HepG2 cell lysate; ; Lane 5 : MCF7 cell lysate; ; Lane 6 : PC3 cell lysate The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1 : Recombinant Human NME1 protein; ; Lane 2 : Recombinant Human NME2 protein; ; Lane 3 : Recombinant Human NME3 protein; ; Lane 4 : Recombinant Human NME4 protein{ATGA0461-WB2.jpg}

ICC/IF analysis of NME1 in HeLa cells. The cell was stained with ATGA0461 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of NME1 in A549 cells. The cell was stained with ATGA0461 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0461-IF2.jpg}