Humaan NM23-H2/NME2-antilichaam

NME2, also known as NM23B, is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. NME1 and NME2 comprise the 152 amino acid A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit of human erythrocyte NDP kinase. NDP kinases are involved in the synthesis of nucleoside triphosphates, and NM23 may act in the regulation of signal transduction by complexing with G proteins, causing activation/inactivation of developmental pathways.

NME/NM23 nucleoside diphosphate kinase 2, Nucleoside diphosphate kinase B, C-myc purine-binding transcription factor PUF, Histidine protein kinase NDKB, NDK B, NDP kinase B, NM23B, NDPKB

Human

Human

Recombinant human NME2 (1-152aa) purified from E. coli

AT2E3

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

NM23-H2, ATGA0475-10 µg, ATGA0475-20 µg, ATGA0475-50 µg, ATGA0475-100 µg, ATGA0475-250 µg, ATGA0475-500 µg, ATGA0475-1 mg, ATGA0475-10, ATGA0475-20, ATGA0475-50, ATGA0475-100, ATGA0475-250, ATGA0475-500, ATGA0475-1

Munkonge FM., et al. (2009) J Biol Chem. 284 (39) : 26978-87.; ; Treharne KJ., et al. (2009) FEBS Lett. 583 (17) : 2789-92.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_001018149

P22392

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: A549 cell lysate; ; Lane 3.: MCF7 cell lysate; ; Lane 4.: LnCap cell lysate The cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: NME2 transfected 293T cell lysate {ATGA0475-WB2.jpg}

ICC/IF analysis of NME2 in HeLa cells. The cell was stained with ATGA0475 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .