Humaan NPM1-antilichaam

Nucleophosmin (NPM; also known as B23, NO38, or numatrin) is a nucleolar phosphoprotein that binds the tumor suppressors p53 and p19Arf and is thought to be indispensable for ribogenesis, cell proliferation, and survival after DNA damage. Nucleophosmin is upregulated in many cancer cells.

Nucleophosmin (nucleolar phosphoprotein B23, numatrin), B23, NPM, MGC104254, nucleolar phosphoprotein B23, numatrin, nucleophosmin/nucleoplasmin family, member 1

Human

Human

Recombinant human NPM (81-294aa) purified from E. coli

5000

Unconjugated

ELISA, WB, ICC/IF, IHC, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

NPM1, ANP0626-10 µg, ANP0626-20 µg, ANP0626-50 µg, ANP0626-100 µg, ANP0626-250 µg, ANP0626-500 µg, ANP0626-1 mg, ANP0626-10, ANP0626-20, ANP0626-50, ANP0626-100, ANP0626-250, ANP0626-500, ANP0626-1

Maiguel DA et al., (2004) Mol cell Biol 24:3703-3711.; ; Colombo Emanuela et al., (2005) Mol cell Biol 25:8874-8886.

Huang M, et al. Autophagy mediates proteolysis of NPM1 and HEXIM1 and sensitivity to BET inhibition in AML cells. (Oncotarget. 2016) {https://pubmed.ncbi.nlm.nih.gov/27732946/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_002511

P06748

The HeLa and HepG2 cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NPM (1:1,000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

This image shows paraffin-embedded human palatine tonsil tissue sample stained with anti-NPM antibody (5E3) at 1:100 dilution.

Flow cytometry analysis of NPM in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of NPM in HeLa cells. The cell was stained with ANP0626 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .