Humaan NPM1-antilichaam

NPM1 is associated with nucleolar ribonucleoprotein structures and bind single-stranded and double-stranded nucleic acids, but it binds preferentially G-Quadruplex forming nucleic acids. It is involved in the biogenesis of ribosomes and may assist small basic proteins in their transport to the nucleolus. Its regulation through SUMOylation (by SENP3 and SENP5) is another facet of the proteins's regulation and cellular functions. It is located in the nucleolus, but it can be translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. The protein is phosphorylated. Chromosomal aberrations involving NPM1 were found in patients with non-Hodgkin lymphoma, acute promyelocytic leukemia, myelodysplastic syndrome, and acute myelogenous leukemia. It has been found in the cytoplasm in patients with primary acute myelogenous leukemia.

Nucleophosmin, B23, NPM

Human

Human

Recombinant human NPM1 (1-294aa) purified from E. coli

AT23F1

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

NPM1, ATGA0345-10 µg, ATGA0345-20 µg, ATGA0345-50 µg, ATGA0345-100 µg, ATGA0345-250 µg, ATGA0345-500 µg, ATGA0345-1 mg, ATGA0345-10, ATGA0345-20, ATGA0345-50, ATGA0345-100, ATGA0345-250, ATGA0345-500, ATGA0345-1

Maiguel D.A., et al. (2004) Mol cell Biol. 24: 3703-3711.; ; Colombo E., et al. (2005) Mol cell Biol. 25: 8874-8886.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Epigenomics (Transcription & Translation)

NP_002511

P06748

The recombinant protein (50ng), cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NPM1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human NPM1 protein; ; Lane 2.: Jurkat cell lysate; ; Lane 3.: 293T cell lysate; ; Lane 4.: HeLa cell lysate; ; Lane 5.: HepG2 cell lysate;

Flow cytometry analysis of NPM1 in WiDr cells. The cell was stained with ATGA0345 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

ICC/IF analysis of NPM1 in WiDr cells. The cell was stained with ATGA0345 (1:200) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .