Humaan NSD3-antilichaam

This gene encoding NSD3 is related to the Wolf-Hirschhorn syndrome candidate - 1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. NSD3 is Histone methyltransferase which preferentially methylates 'Lys-4' and 'Lys-27' of histone H3 and is amplified in several tumor cell lines and primary breast carcinomas.

Transcription elongation factor B polypeptide 3 binding protein 1, DKFZp667H044, FLJ20353, Histone lysine N methyltransferase NSD3, MGC126766, MGC142029, NSD 3, Nuclear set domain containing 3, Nuclear SET domain containing protein 3, pp14328, Whistle, WHSC1 like protein 1, WHSC1L1 protein, Wolf Hirschhorn syndrome candidate 1 like 1

Human

Human

Recombinant human NSD3 (383-660aa) purified from E. coli

AT7B4

Unconjugated

ELISA, WB

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

NSD3, Transcription elongation factor B polypeptide 3 binding protein 1, DKFZp667H044, FLJ20353, Histone lysine N methyltransferase NSD3, MGC126766, MGC142029, NSD 3, Nuclear set domain containing 3, Nuclear SET domain containing protein 3, pp14328, Whistle, WHSC1 like protein 1, WHSC1L1 protein, Wolf Hirschhorn syndrome candidate 1 like 1, ANS0804-10 µg, ANS0804-20 µg, ANS0804-50 µg, ANS0804-100 µg, ANS0804-250 µg, ANS0804-500 µg, ANS0804-1 mg, ANS0804-10, ANS0804-20, ANS0804-50, ANS0804-100, ANS0804-250, ANS0804-500, ANS0804-1

Angrand P.O, et al. (2001) Genomics 74 (1) :79-88; ; Stec I, et al. (2000) FEBS Lett 473 (1) :1-5

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_075447

Q9BZ95

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSD3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: HeLa cell lysate ; ; Lane 3.: MCF7 cell lysate ; ; Lane 4.: NIH3T3 cell lysate ; ; Lane 5.: U87MG cell lysate