Humaan OAT-antilichaam

OAT is a 49-kDa nucleus-encoded protein imported into mitochondria to give the mature 48-kDa OAT polypeptide. It has been described in humans, animals, insects, plants and microorganisms. Especially OAT have sex-differential expression in the mouse kidney. OAT plays crucial physiological roles in amino acid metabolism. OAT shows a large structural and mechanistic similarity to other enzymes from the subgroup III of aminotransferases, which transfer an amino group from a carbon atom that does not carry a carboxyl function. OAT is essential for nitrogen recycling from arginine but not for the stress-induced proline accumulation.

Ornithine aminotransferase precursor, DKFZp781A11155, HOGA, OATASE, ornithine aminotransferase precursor EC 2.6.1.13, ornithine aminotransferase (gyrate atrophy), ornithine aminotransferase precursor, Ornithine aminotransferase, mitochondrial precursor, Ornithine oxo-acid aminotransferase, Ornithine--oxo-acid aminotransferase

Human

Human

Recombinant human OAT (33-439aa) purified from E.coli

AT23A2

Unconjugated

ELISA, WB, ICC/IF

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

OAT, ATGA0176-10 µg, ATGA0176-20 µg, ATGA0176-50 µg, ATGA0176-100 µg, ATGA0176-250 µg, ATGA0176-500 µg, ATGA0176-1 mg, ATGA0176-10, ATGA0176-20, ATGA0176-50, ATGA0176-100, ATGA0176-250, ATGA0176-500, ATGA0176-1

Stranska J, et al. (2008) Plant Signal Behav, 3 (11) :929-35.; ; Canas RA, et al. (2008) Plant Physiol, 148 (1) :77-88.; ; Levillain O, et al. (2007) Am J Physiol Renal Physiol, 292 (3) :F1016-27.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Metabolism

NP_000265

P04181

Tissue lysate of mouse liver (35ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human OAT (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The recombinant protein (20ng) and lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human OAT antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: OAT recombinant protein; ; Lane 2.: 293T cell lysate; ; Lane 3.: Mouse brain tissue lysate {ATGA0176-WB2.jpg}

ICC/IF analysis of OAT in HeLa cells. The cell was stained with ATGA0176 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of OAT in Hep3B cells. The cell was stained with ATGA0176 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0176-IF2.jpg}