Humaan Otubain-1/OTUB1-antilichaam

Otubain 1, also known as OTUB1, is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases and inhibits cytokine gene transcription in the immune system via its interaction with an ubiquitin protease and E3 ubiquitin ligase. This protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function of polyubiquitin chain growth.

Ubiquitin thioesterase Otubain 1, OTB1, OTu1, ubiquitin thioesterase Otubain 1 Deubiquitinating enzyme OTuB1, OTu domain containing ubiquitin aldehyde binding protein 1, Otubain 1, ubiquitin specific processing protease OTuB1, ubiquitin thioesterase OTuB1.

Human

Human

Recombinant human OTUB1 (1-271aa) purified from E. coli

AT17E10

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Otubain1, OTUB1, ubiquitin thioesterase Otubain 1, OTB1, OTu1, ubiquitin thioesterase Otubain 1 Deubiquitinating enzyme OTuB1, OTu domain containing ubiquitin aldehyde binding protein 1, Otubain 1, ubiquitin specific processing protease OTuB1, ubiquitin thioesterase OTuB1., ATGA0380-10 µg, ATGA0380-20 µg, ATGA0380-50 µg, ATGA0380-100 µg, ATGA0380-250 µg, ATGA0380-500 µg, ATGA0380-1 mg, ATGA0380-10, ATGA0380-20, ATGA0380-50, ATGA0380-100, ATGA0380-250, ATGA0380-500, ATGA0380-1

Balakirev MY., et al. (2003) EMBO Rep. 4 (5) : 5175-5122.; ; Soares L., et al. (2004) Nat Immunol. 5 (1) : 45-54.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Enzymes & Proteases

NP_060140

Q96FW1

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human OTUB1 antibody (1:5000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : A549 cell lysate ; ; Lane 2. : HeLa cell lysate ; ; Lane 3. : HepG2 cell lysate ; ; Lane 4. : 293T cell lysate

ICC/IF analysis of OTUB1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human OTUB1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .