Humaan Otubain-2/OTUB2-antilichaam

OTUB2 (Ubiquitin thioesterase OTUB2) belongs to the peptidase C65 family. OTUB2 functions as a hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation.

Ubiquitin thioesterase OTuB2, OTB2, OTu2, TimntanOTUB2 aUgitin thioesterase 2

Human

Human

Recombinant human OTUB2 (1-234aa) purified from E. coli

AT1F8

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

OTUB2, ATGA0511-10 µg, ATGA0511-20 µg, ATGA0511-50 µg, ATGA0511-100 µg, ATGA0511-250 µg, ATGA0511-500 µg, ATGA0511-1 mg, ATGA0511-10, ATGA0511-20, ATGA0511-50, ATGA0511-100, ATGA0511-250, ATGA0511-500, ATGA0511-1

Balakirev MY, et al. (2003) EMBO Rep 4 (5) : 517-22.; ; Edelmann M.J., et al. (2009) Biochem. J. 418:379-390.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Ubiquitination

NP_075601

Q96DC9

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human OTUB2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: OTUB2 transfected 293T cell lysate