Humaan pan-RAS antilichaam

The Ras protein is a subfamily of small GTPases that are involved in signal transduction. They are activated by ligand-binding receptor tyrosine kinases such as EGFR, PDGFR, CSF and FGF. These kinases transiently convert RAS-GDP (inactive form) to RAS-GTP, the active form of RAS. The main genes are HRAS, NRAS and KRAS.

Neuroblastoma RAS viral (v-ras) oncogene homolog, GTPase NRas, HRAS1, ALPS4, N-ras, NRAS1, NS6, Neuroblastoma RAS viral (v-ras) oncogene homolog OTTMuSP00000023521, AV095280, N ras, N ras protein part 4, Neuroblastoma RAS viral (v ras) oncogene homolog, OTTHuMP00000013879, Transforming protein N Ras, v ras neuroblastoma RAS viral oncogene homolog

Human

Human

Recombinant human NRAS (1-186aa) purified from E. coli

AT2G9

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

V ras neuroblastoma RAS viral oncogene homolog, Transforming protein N Ras, OTTHuMP00000013879, NS6, NRAS1, N-ras, Neuroblastoma RAS viral (v-ras) oncogene homolog OTTMuSP00000023521, Neuroblastoma RAS viral (v-ras) oncogene homolog, OTTMuSP00000023521, Neuroblastoma RAS viral (v-ras) oncogene homolog, Neuroblastoma RAS viral (v ras) oncogene homolog, N ras protein part 4, N ras, HRAS1, GTPase NRas, AV095280, ALPS4, RAS, ATGA0203-10 µg, ATGA0203-20 µg, ATGA0203-50 µg, ATGA0203-100 µg, ATGA0203-250 µg, ATGA0203-500 µg, ATGA0203-1 mg, ATGA0203-010, ATGA0203-020, ATGA0203-050, ATGA0203-100, ATGA0203-250, ATGA0203-500, ATGA0203-01M

Rocks O, et al. (2006) . Current Opinion in Cell Biology 18 (4) : 351-7.; ; Wennerberg K, et al. (March 2005) . J. Cell. Sci. 118 (Pt 5) : 843-6.; ; Rotblat B, et al. (2008) . Methods Enzymol 439: 467-89.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_002515

P01111

The cell lysates of 293T (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS (1:500~1:5000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The recombinant proteins (200ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human NRAS protein; ; Lane 2.: Recombinant human KRAS protein; ; Lane 3.: Recombinant human HRAS protein; ; {ATGA0203-WB2.jpg}

Flow cytometry analysis of RAS in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of RAS in HeLa cells. The cell was stained with ATGA0203 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .