Humaan pan-RAS antilichaam

The Ras protein is a subfamily of small GTPases that are involved in signal transduction. They are activatedby ligand-binding receptor tyrosine kinases such as EGFR, PDGFR, CSF and FGF. These kinasestransiently convert RAS-GDP (inactive form) to RAS-GTP, the active form of RAS. The main genes areHRAS, NRAS and KRAS.

Neuroblastoma RAS viral (v-ras) oncogene homolog, GTPase NRas, HRAS1, ALPS4, N-ras, NRAS1, NS6, Neuroblastoma RAS viral (v-ras) oncogene homolog OTTMuSP00000023521, AV095280, N ras, N rasprotein part 4, Neuroblastoma RAS viral (v ras) oncogene homolog, OTTHuMP00000013879, Transforming protein N Ras, v ras neuroblastoma RAS viral oncogene homolog

Human

Human

Recombinant human NRAS (1-186aa) purified from E. coli

AT2G9

Unconjugated

ELISA, WB, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

RAS, ATGA0573-10 µg, ATGA0573-20 µg, ATGA0573-50 µg, ATGA0573-100 µg, ATGA0573-250 µg, ATGA0573-500 µg, ATGA0573-1 mg, ATGA0573-10, ATGA0573-20, ATGA0573-50, ATGA0573-100, ATGA0573-250, ATGA0573-500, ATGA0573-1

Rocks O, et al. (2006) . Current Opinion in Cell Biology 18 (4) : 351-7.; ; Wennerberg K, et al. (March 2005) . J. Cell. Sci. 118 (Pt 5) : 843-6.; ; Rotblat B, et al. (2008) . Methods Enzymol 439: 467-89.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cancer

NP_002515

P01111

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human NRAS protein; ; Lane 2.: Recombinant human KRAS protein; ; Lane 3.: Recombinant human HRAS protein; ; The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; {2004010146591329.jpg}

Flow cytometry analysis of RAS in HeLa cells. The cell was stained with ATGA0573 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .