Humaan PARP2-antilichaam

PARP2 is poly (ADP-ribosyl) transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly (ADP-ribosyl) ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found.

Poly (ADP-ribose) polymerase 2, ADPRT2, ADPRTL2, ADPRTL3, ARTD2, pADPRT-2, PARP-2

Human

Human

Recombinant human PARP2 (233-583aa) purified from E. coli

AT29G4

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

PARP2, ATGA0270-10 µg, ATGA0270-20 µg, ATGA0270-50 µg, ATGA0270-100 µg, ATGA0270-250 µg, ATGA0270-500 µg, ATGA0270-1 mg, ATGA0270-10, ATGA0270-20, ATGA0270-50, ATGA0270-100, ATGA0270-250, ATGA0270-500, ATGA0270-1

Ame JC, Rolli V, Schreiber V et al. (1999) . J. Biol. Chem. 274 (25) : 17860-8.; ; Schreiber V, Ame JC, Dolle P et al. (2002) . J. Biol. Chem. 277 (25) : 23028-36.; ; Maeda Y, Hunter TC, Loudy DE et al. (2006) . J. Biol. Chem. 281 (14) : 9600-6.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Epigenomics (Transcription & Translation)

NP_005475

Q9UGN5

The cell lysate of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:500 ~ 1:5000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : Raji cell lysate; ; Lane 2. : NIH-3T3 cell lysate; ; {ATGA0270-WB2.jpg}

Flow cytometry analysis of PARP2 in U87MG cells. The cell was stained with ATGA0270 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black) .

ICC/IF analysis of PARP2 in HeLa cells. The cell was stained with ATGA0270 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .