Humaan PD-1 (Programmed Cell Death Protein1) Voorverpakte ELISA Kit
Kwaliteit
ISO Gecertificeerd
Levering
24-48 uur
Technische Specificaties
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human PD-1 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human PD-1 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PD-1 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human PD-1 can be calculated using the standard curve.
ELISA
1 x 96 well Format (96 Tests)
Please refer to the Manual
https://www.abeomics.com/images/90-2111/Human%20PD-1_Manual.pdf
This immunoassay kit allows for the in vitro quantitative determination of Human PD-1 concentrations in serum, plasma and other biological fluids. Detection Range: 0.156 - 10 ng/ml Sensitivity: < 0.094 ng/ml
Beschrijving
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human PD-1 antibody was pre-coated into 96-well plates. Biotin conjugated an
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