Humaan PDCL3-antilichaam

PDCL3 (phosducin-like 3), also known as VIAF1 (viral IAP-associated factor 1), VIAF, PHLP3 or HTPHLP, is a widely expressed protein that belongs to the phosducin-like family of proteins. phosducin-like family members associate with the cytosolic chaperonin complex (CCT) and regulate the folding of proteins. Localizing to the cytoplasm, PDCL3 does not appear to play a role in G protein signaling, but participates in the chaperone-assisted folding of proteins, such as beta Tubulin and Actin, that are involved in the regulation of cell cycle progression. More specifically, PDCL3, when associated with CCT, represses the ATPase activity of CCT and ultimately disrupts the folding of the Actin or tubulin substrates.

Phosducin-Like protein 3, Phosducin-Like protein 3, HTPHLP, MGC3062, PHLP2A, PHLP3, VIAF, VIAF1

Human

Human

Recombinant human PDCL3 (1-239aa) purified from E. coli

AT8F9

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

PDCL3, ATGA0333-10 µg, ATGA0333-20 µg, ATGA0333-50 µg, ATGA0333-100 µg, ATGA0333-250 µg, ATGA0333-500 µg, ATGA0333-1 mg, ATGA0333-10, ATGA0333-20, ATGA0333-50, ATGA0333-100, ATGA0333-250, ATGA0333-500, ATGA0333-1

Wilkinson J.C., et al. (2004) Biol. Chem. 279 (49) :51091-51099.; ; Hillier L.W., et al. (2005) Nature 434 (7034) :724-731.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.

Signal Transduction

NP_076970

Q9H2J4

The cell lysates of Jurkat (40ug), A549 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDCL3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Jurkat cell lysate ; ; Lane 2.: A549 cell lysateThe Recombinant protein (100ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDCL3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Recombinant Protein PDCL3 100ng{ATGA0333-WB2.jpg}

ICC/IF analysis of PDCL3 in HeLa cells. The cell was stained with ATGA0333 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of PDCL3 in PC3 cells. The cell was stained with ATGA0333 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0333-IF2.jpg}