Humaan PDHX antilichaam

The pyruvate dehydrogenase (PDH) complex is located in the mitochondrial matrix and catalyzes the conversion of pyruvate to acetyl coenzyme A. The PDH complex thereby links glycolysis to Krebs cycle. The PDH complex contains three catalytic subunits, E1, E2, and E3, two regulatory subunits, E1 kinase and E1 phosphatase, and a non-catalytic subunit, E3 binding protein (E3BP) . This gene encodes the E3 binding protein subunit; also known as component X of the pyruvate dehydrogenase complex. This protein tethers E3 dimers to the E2 core of the PDH complex.

Pyruvate dehydrogenase protein X component mitochondrial, Pyruvate dehydrogenase protein X component, mitochondrial, DLDBP, E3BP, OPDX, PDX1, proX

Human

Human

Recombinant human PDHX (54-501aa) purified from E. coli.

AT1E11

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

PDHX, Pyruvate dehydrogenase protein X component mitochondrial, Pyruvate dehydrogenase protein X component, mitochondrial, DLDBP, E3BP, OPDX, PDX1, proX, ATGA0494-10 µg, ATGA0494-20 µg, ATGA0494-50 µg, ATGA0494-100 µg, ATGA0494-250 µg, ATGA0494-500 µg, ATGA0494-1 mg, ATGA0494-10, ATGA0494-20, ATGA0494-50, ATGA0494-100, ATGA0494-250, ATGA0494-500, ATGA0494-1

Aral B., et al. (1997) Am. J. Hum. Genet. 61: 1318-1326.; ; Hiromasa Y., et al. (2004) J. Biol. Chem. 279: 6921-6933.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_003468

O00330

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDHX antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: A431 cell lysate ; ; Lane 2.: HepG2 cell lysate ; ; Lane 3.: 293T cell lysate ; ; Lane 4.: LnCap cell lysate