Humaan PDZK1-antilichaam

PDZK1 is PDZ domain-containing scaffolding protein with a 519 amino acid protein. PDZK1 mediates the localization of cell surface proteins and plays a critical role in cholesterol metabolism by regulating the HDL receptor, scavenger receptor class B type 1. This protein is expressed in the kidney, pancreas, liver, gastrointestinal tract, and adrenal cortex. In situ hybridization experiments showed that the expression of PDZK1 was limited to epithelial cells. In addition, PDZK1 was overexpressed in selected tumors of epithelial origin.

Na (+) /H (+) exchange regulatory cofactor NHE-RF3, Na (+) /H (+) exchange regulatory cofactor NHE-RF3, CAP70, CLAMP, NHERF-3, NHERF3, PDZD1

Human

Human

Recombinant human PDZK1 (1-519aa) purified from E. coli

AT1A2

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

PDZK1, ATGA0243-10 µg, ATGA0243-20 µg, ATGA0243-50 µg, ATGA0243-100 µg, ATGA0243-250 µg, ATGA0243-500 µg, ATGA0243-1 mg, ATGA0243-10, ATGA0243-20, ATGA0243-50, ATGA0243-100, ATGA0243-250, ATGA0243-500, ATGA0243-1

Kocher, O., et al. (1999) Lab Invest 64: 1733-1745.; ; Kocher, O., et al. (2001) Mol Cell Biol 23: 1175-1180.; ; Gisler, S.M., et al (2003) Kidney Int 64 (5) : 1733-45.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Metabolism

NP_001188254

Q5T2W1

The cell lysates of HeLa (40ug) and 293T (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDZK1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Flow cytometry analysis of PDZK1 in Hep3B cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of PDKZ1 in Hep3B cells. The cell was stained with ATGA0243 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .