Humaan PEDFR/PNPLA2-antilichaam

Adipose triglyceride lipase (ATGL) is a 504 amino acid protein that is highly expressed in mouse and human adipose tissue. ATGL is catalyzes the initial step in triglyceride hydrolysis in adipocyte lipid droplets and has acylglycerol transacylase activity. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity. Thus, ATGL and hormone-sensitive lipase coordinately catabolize stored triglycerides in adipose tissue of mammals.

Adipose triglyceride lipase, Calcium-independent phospholipase A2, Desnutrin IPLA2-zeta, Pigment epithelium-derived factor receptor, TTS2.2, Transport-secretion protein 2, TTS2, ATGL, Patatin-like phospholipase domain-containing protein 2, PNPLA2

Human

Human

Recombinant human ATGL (30-504aa) purified from E. coli

AT18E6

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

TTS2.2, TTS2, Transport-secretion protein 2, PNPLA2, Pigment epithelium-derived factor receptor, PEDF PNPLA2, Patatin-like phospholipase domain-containing protein 2, DesnutrinIPLA2-zeta, Desnutrin IPLA2-zeta, Calcium-independent phospholipase A2, ATGL, Adipose triglyceride lipase, ATGA0122-10 µg, ATGA0122-20 µg, ATGA0122-50 µg, ATGA0122-100 µg, ATGA0122-250 µg, ATGA0122-500 µg, ATGA0122-1 mg, ATGA0122-010, ATGA0122-020, ATGA0122-050, ATGA0122-100, ATGA0122-250, ATGA0122-500, ATGA0122-01M

Fischer J, et al., (2007) Nat Genet. 39 (1) : 28-30.; ; Elena S, et al., (2006) EMBO Rep. 7 (1) : 106-113.; ; Zimmermann R, et al., (2004) Science. 306 (5700) : 1383-1386.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cardiovascular

NP_065109

Q96AD5

The A431 cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATGL antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The mouse adipose tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATGL antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. {ATGA0122-WB2.jpg}

Flow cytometry analysis of ATGL in Hep3B cells. The cell was stained with ATGA0122 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .Flow cytometry analysis of ATGL in HeLa cells. The cell was stained with ATGA0122 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .{2004010103833302.jpg}

ICC/IF analysis of ATGL in Hep3B cells. The cell was stained with ATGA0122 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of ATGL in HeLa cells. The cell was stained with ATGA0122 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{2004010103622287.jpg}ICC/IF analysis of ATGL in A549 cells. The cell was stained with ATGA0122 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{2004010103476044.jpg} {ATGA0122-IF3b.jpg}