Humaan PEDF/SERPINF1-antilichaam

Pigment epithelium-derived factor (PEDF) is a 50kDa glycoprotein and member of the serpin superfamily without any activity of serine protease. PEDF is an angiogenesis inhibitor with neurotrophic properties, balances angiogenesis in the eye and blocks tumor progression. That is, in neuronal cell, PEDF is promoting cell survival and differentiation, however, when endothelial cells are exposed to PEDF, their cell migration and proliferation are inhibited, and they undergo apoptosis.

SERPINF1, Serpin peptidase inhibitor clade F member 1, Proliferation inducing protein 35, Pigment epithelium-derived factor EPC 1, Pigment epithelium-derived factor, PIG35, PI, GSTP1, GST3, Glutathione S-Transferase Pi 1, Glutathione S-transferase P Fatty Acid Ethyl Ester Synthase III, Glutathione S-transferase P, Glutathione S Transferase Pi, FAEES3, EPC1, DFN7, APF0619

Human

Human

Recombinant human PEDF (20-418aa) purified from E. coli.

AT13D9

Unconjugated

ELISA, WB, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

EPC1, EPC-1, Glutathione S-Transferase pi1, GSTP1, IL 1 A, IL 1 alpha, IL 1A, IL1 alpha, IL-1 alpha, IL1A, IL-1A, IL1alpha, IL1F1, Interleukin 1 alpha, Interleukin 1-alpha, Interleukin-1 alpha, Interleukin-1 alpha precursor, PEDF, PIG 35, PIG35, Pigment epithelium derived factor, Pigment epithelium derived factor EPC 1, Pigment epithelium-derived factor, Pigment epithelium-derived factor EPC 1, Proliferation inducing protein 35, Serpin F1, Serpin peptidase inhibitor clade F member 1, SERPINF 1, SERPINF1, SERPIN-F1, ATGA0484-10 µg, ATGA0484-20 µg, ATGA0484-50 µg, ATGA0484-100 µg, ATGA0484-250 µg, ATGA0484-500 µg, ATGA0484-1 mg, ATGA0484-010, ATGA0484-020, ATGA0484-050, ATGA0484-100, ATGA0484-250, ATGA0484-500, ATGA0484-01M

Baba H. et al (2005) Arteriosler Thromb Vasc Biol. 25: 1938-1944.; ; Filleur S. et al (2005) Cancer Res. 65: 5144-5152.; ; Hattenbach LO. et al (2004) Ophthalmic Res. 37: 341-346.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Hormones & Cytokines

NP_002606.3

P36955

The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PEDF antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: PEDF transfected 293T cell lysate

Flow cytometry analysis of PEDF in K562 cells. The cell was stained with ATGA0484 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .