Humaan PGK1-antilichaam

Phosphoglycerate kinase 1 (PGK1) is an X-linked enzyme that plays a key role in the glycolytic pathway. The gene encoding the erythrocyte enzyme PGK1 and it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) as a glyxolytic enzyme role. The PGK1 catalyzes the reversible conversion of 1, 3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP.

Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2, PGKA, MIG10

Human

Human

Recombinant human PGK1 (1-417aa) purified from E. coli

AT2F4

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

PGK1, Phosphoglycerate kinase 1, Pgk-1, ATGA0381-10 µg, ATGA0381-20 µg, ATGA0381-50 µg, ATGA0381-100 µg, ATGA0381-250 µg, ATGA0381-500 µg, ATGA0381-1 mg, ATGA0381-10, ATGA0381-20, ATGA0381-50, ATGA0381-100, ATGA0381-250, ATGA0381-500, ATGA0381-1

Svaasand EK., et al. (2007) Muscle Nerve. 36 (5) : 679-684. ; ; Valentin C., et al. (1998) Hum Mutat. 12 (4) : 280-287.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_000282

P00558

Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGK1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : HeLa cell lysate ; ; Lane 2. : LNCaP cell lysate ; ; Lane 3. : A549 cell lysate ; ; Lane 4. : HepG2 cell lysate ; ; Lane 5. : 293T cell lysate ; ; Lane 6. : K562 cell lysate ; ; Lane 7. : Jurkat cell lysate

ICC/IF analysis of PGK1 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PGK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .