Humaan PGM1-antilichaam

PGM1 is an evolutionarily conserved enzyme that regulates one of the most important metabolic carbohydrate trafficking points in prokaryotic and eukaryotic organisms, catalyzing the bi-directional interconversion of glucose 1-phosphate (G-1-P) and glucose 6-phosphate (G-6-P) . In one direction, G-1-P produced from sucrose catabolism is converted to G-6-P, the first intermediate in glycolysis. In the other direction, conversion of G-6-P to G-1-P generates a substrate for synthesis of UDP-glucose, which is required for synthesis of a variety of cellular constituents, including cell wall polymers and glycoproteins. PGM1 has been used extensively as a genetic marker for isozyme polymorphism among humans. PGM is known to be post-translationally modified by cytoplasmic glycosylation that does not seem to regulate its enzymatic activity but rather is implicated in the localization of the protein. Glucose 1, 6 bisphosphate (Glc-1, 6-P2), a powerful regulator of carbohydrate metabolism, has been demonstrated to be a potent activator of PGM. PGM1 is also modified by phosphorylation on Ser108 as part of its catalytic mechanism. This is shown to be performed by Pak1, a previously identified signaling kinase.

Phosphoglucomutase-1 isoform 1, CDG1T, GSD14

Human

Human

Recombinant human PGM1 (1-562aa) purified from E. coli

AT84G2

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

PGM1, ATGA0406-10 µg, ATGA0406-20 µg, ATGA0406-50 µg, ATGA0406-100 µg, ATGA0406-250 µg, ATGA0406-500 µg, ATGA0406-1 mg, ATGA0406-10, ATGA0406-20, ATGA0406-50, ATGA0406-100, ATGA0406-250, ATGA0406-500, ATGA0406-1

Boros LG., et al. (2002) Pancreas. 24 (1) : 26-33.; ; Dey NB., et al. (1994) The Journal of Biological Chemistry. 269 (43) : 27143-8.; ; Gururaj A., et al. (2004) Oncogene. 23 (49) : 8118-27.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Metabolism

NP_002624

P36871

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGM1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : 293T cell lysate; ; Lane 2. : HepG2 cell lysate ; ; Lane 3. : NIH/3T3 cell lysate; ; Lane 4. : Jurkat cell lysate; ; Lane 5. : HeLa cell lysate; ; Lane 6. : U87MG cell lysate ;

ICC/IF analysis of PGM1 in HeLa cells. The cell was stained with ATGA0406 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .