Humaan PIN/DYNLL1-antilichaam

The LC8 family members of dynein light chains (DYNLL1 and DYNLL2 in vertebrates) are highly conserved ubiquitous eukaryotic homodimer proteins that interact, besides dynein and myosin 5a motor proteins, with a large (and still incomplete) number of proteins involved in diverse biological functions.

Dynein light chain 1 cytoplasmic, DLC1, DLC8, DNCL1, DNCLC1, hdlc1, LC8, LC8a, MGC126137, MGC126138, PIN, 8 kDa dynein light chain, Dynein light chain LC8-type 1, Protein inhibitor of neuronal nitric oxide synthase

Human

Human

Recombinant human DYNLL (1-89aa) purified from E. coli

AT13C9

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

PIN, DYNLL, Dynein light chain 1 cytoplasmic, DLC1, DLC8, DNCL1, DNCLC1, hdlc1, LC8, LC8a, MGC126137, MGC126138, PIN, ATGA0383-10 µg, ATGA0383-20 µg, ATGA0383-50 µg, ATGA0383-100 µg, ATGA0383-250 µg, ATGA0383-500 µg, ATGA0383-1 mg, ATGA0383-10, ATGA0383-20, ATGA0383-50, ATGA0383-100, ATGA0383-250, ATGA0383-500, ATGA0383-1

Rapali P., et al. (2011) FEBS J. 278 (17) : 2980-2996.; ; Radnai L., et al. (2010) J Biol Chem. 285 (49) : 38649-38657.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Apoptosis

NP_003737

P63167

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DYNLL antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : HeLa cell lysate ; ; Lane 2. : A549 cell lysate ; ; Lane 3. : 293T cell lysate ; ; Lane 4. : HepG2 cell lysate

ICC/IF analysis of DYNLL in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human DYNLL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .