Humaan PKM antilichaam

PKM2 (pyruvate kinase M2), its association with phospho-tyrosine (pTyr) motifs, enables cells to use predominantly aerobic glycolysis instead of oxidative phosphorylation. Also PKM2 suggest that this change benefits cancer cells by enabling them to redirect glycolytic metabolites away from oxidation and energy production and, instead, toward anabolic processes and biosynthesis. PKM2-mediated generation of the aerobic glycolytic phenotype, although wasteful in terms of glucose consumption and waste generation, could provide a variety of advantages for cancerous cells. In addition nuclear translocation of the tumor marker pyruvate kinase M2 induces programmed cell death.

Pyruvate kinase muscle isoform M2, Pyruvate kinase M1/2, Pyruvate kinase muscle, Cytosolic thyroid hormone-binding protein, CTHBP, Pyruvate kinase 2/3, Threonine-protein kinase PKM2, Thyroid hormone-binding protein 1, THBP1, Opa-interacting protein 3, OIP3, Tumor M2-PK, Tyrosine-protein kinase PKM2, PK2, PK3, PKM2, p58

Human

Human

Recombinant human PKM2 (1-531aa) purified from E.coli

AT1B10

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

PKM2, pyruvate kinase muscle isoform M2, CTHBP, THBP1, p58, M2-PK, Tumor M2-PK, PK2, PK3, PKM, pyruvate kinase, muscle isoform M2 OIP3, PKM, TCB, OPA interacting protein 3, PKM2, Cytosolic thyroid hormone binding protein, Pyruvate kinase 2/3, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle, Thyroid hormone binding protein cytosolic, Tumour Type M2 Pyruvate Kinase, Tumor Type M2 Pyruvate Kinase, ATGA0325-10 µg, ATGA0325-20 µg, ATGA0325-50 µg, ATGA0325-100 µg, ATGA0325-250 µg, ATGA0325-500 µg, ATGA0325-1 mg, ATGA0325-10, ATGA0325-20, ATGA0325-50, ATGA0325-100, ATGA0325-250, ATGA0325-500, ATGA0325-1

Ferguson E.C., et al. (2008) Trends Biochem Sci 33 (8) : 359-362.; ; Christofk H.R., et al. (2008) Nature 452 (7184) :181-186.; ; Shimada N., et al. (2008) Genes Cells 13 (3) : 245-254.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution for Western blot analysis is 1:1000 and Immunofluorescence is 1:250.

Signal Transduction

NP_002645

P14618

Fig.1: The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKM2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1 : HeLa cell lysate ; ; Lane 2 : Jurkat cell lysate ; ; Lane 3 : MCF7 cell lysate ; ; Lane 4 : A549 cell lysate ; ; Lane 5 : 293T cell lysate Fig 2: The Recombinant human PKM2 (50ng) protein was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKM2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. {ATGA0325-WB2.jpg}

ICC/IF analysis of PKM2 in HeLa cells. The cell was stained with ATGA0325 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of PKM2 in A549 cells. The cell was stained with ATGA0325 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{2004010268900815.jpg}