Humaan PMVK antilichaam

Phosphomevalonate kinase, also known as PMVK, is a cytosolic enzyme. It was expressed highly in heart, liver, skeletal muscle, kidney and pancreas and slightly lower in brain, placenta, and lung. Induced by sterol, PMVK participates in isopentenyl diphosphate biosynthesis via the mevalonate pathway. PMVK catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate in the fifth reaction of the cholesterol biosynthetic pathway.

Phosphomevalonate kinase, HuMPMKI, PMK, PMKA, PMKASE, Phosphomevalonate kinase

Human

Human

Recombinant human PMVK (1-192aa) purified from E. coli

AT21C10

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

PMVK, ATGA0349-10 µg, ATGA0349-20 µg, ATGA0349-50 µg, ATGA0349-100 µg, ATGA0349-250 µg, ATGA0349-500 µg, ATGA0349-1 mg, ATGA0349-10, ATGA0349-20, ATGA0349-50, ATGA0349-100, ATGA0349-250, ATGA0349-500, ATGA0349-1

Cho YK., et al. (2001) J Biol chem. 278: 4510-4515.; ; Hogenboom S., et al. (2004) J Lipid Res. 45: 697-705.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cancer

NP_006547

Q15126

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PMVK antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : HeLa cell lysate; ; Lane 2. : HepG2 cell lysate; ; Lane 3. : Jukat cell lysate; ; Lane 4. : MCF7 cell lysate; ; Lane 5. : A549 cell lysate

Flow cytometry analysis of PMVK in LNCap cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of PMVK in PC3 cells. The cell was stained with ATGA0349 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .