Humaan PNMT antilichaam

Phenylethanolamine N-methyltransferase (PNMT) catalyzes the synthesis of epinephrine and plays an important role in regulating cardiovascular function. Expression of PNMT is regulated by hormonal and neural stimuli because its promoter contains sequences responding to cholinergic and depolarization stimuli. PNMT is the final enzyme in the catecholamine pathway, catalyzing the conversion of norepinephrine to epinephrine using as the cosubstrate and methyl donor, S-adenosylmethionine.

Phenylethanolamine N-methyltransferase, PENT, PNMTase, Noradrenaline-N-methyltransferase, Phenylethanolamine N-methyltransferase

Human

Human

Recombinant human PNMT (1-282aa) purified from E. coli

AT1C11

Unconjugated

ELISA, WB, ICC/IF

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

PNMT, ATGA0214-10 µg, ATGA0214-20 µg, ATGA0214-50 µg, ATGA0214-100 µg, ATGA0214-250 µg, ATGA0214-500 µg, ATGA0214-1 mg, ATGA0214-10, ATGA0214-20, ATGA0214-50, ATGA0214-100, ATGA0214-250, ATGA0214-500, ATGA0214-1

Wurtman R.J., et al. (1965) Science 150: 1464-1465.; ; Wurtman R.J., et al. (1966) J Biol Chem 241: 2301-2305.; ; Huang C., et al. (2011) lAmerican Journal of Hypertension 24: 1222-1226.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Metabolism

NP_002677

P11086

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PNMT antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: PNMT transfected 293T cell lysate

ICC/IF analysis of PNMT in Hep3B cells. The cell was stained with ATGA0214 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .