Humaan Pontine/RUVBL1 antilichaam

RuVBL1, also known as TIP49A, is a component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This complex may be required for the activation of transcriptional programs associated with oncogene and proto oncogene mediated growth induction, tumor suppressor mediated growth arrest.

49 kDa TATA box-binding protein-interacting protein, 49 kDa TBP-interacting protein, 54 kDa erythrocyte cytosolic protein, ECP-54, INO80 complex subunit H, INO80H, NMP 238, Nuclear matrix protein 238, Pontin 52, RuvB like AAA ATPase 1, RuvB-like 1, RVB1, TAP54-alpha, TIH1, TIP49a, TIP60-associated protein 54-alpha

Human

Human

Recombinant human RuVBL1 (1-456aa) purified from E. coli

AT1D6

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

49 kDa TATA box-binding protein-interacting protein, 49 kDa TBP-interacting protein, 54 kDa erythrocyte cytosolic protein, ECP 54, ECP54, ECP-54, INO80 complex subunit H, INO80H, NMP 238, NMP238, NMP-238, Nuclear matrix protein 238, PONTIN, Pontin 52, Pontin52, Pontin-52, RuvB like AAA ATPase 1, RuVBL 1, RuVBL1, RuVBL-1, RuvB-like 1, RVB1, TAP54-alpha, TIH1, TIP 49, TIP49, TIP-49, TIP49a, TIP60-associated protein 54-alpha, ATGA0423-10 µg, ATGA0423-20 µg, ATGA0423-50 µg, ATGA0423-100 µg, ATGA0423-250 µg, ATGA0423-500 µg, ATGA0423-1 mg, ATGA0423-010, ATGA0423-020, ATGA0423-050, ATGA0423-100, ATGA0423-250, ATGA0423-500, ATGA0423-01M

Jha S., et al. (2009) Mol Cell. 34 (5) : 521-33.; ; Haurie V., et al. (2009) Hepatology. 50 (6) : 1871-83.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Epigenomics (Transcription & Translation)

NP_003698

Q9Y265

The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RuVBL1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1 : Raji cell lysate

ICC/IF analysis of RuVBL in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RuVBL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0423-IF1b.jpg}