Humaan PP1 Katalytisch Subeenheid/PPP1CA antilichaam

Protein phosphatase 1, catalytic subunit, alpha isoform, also known as PPP1CA, is one of the three catalytic subunits of protein phosphatase 1. The protein is highly conserved in eukaryotic cells and play a role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. It is participate in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis and apoptosis.

Serine/threonine-protein phosphatase PP1-alpha catalytic subunit isoform 1, protein phosphatase 1 catalytic subunit alpha isozyme, PP-1A, PP1alpha, ppp1a, MGC15877, MGC1674

Human

Human

Recombinant human PPP1CA (30-299aa) purified from E. coli

4G3

Unconjugated

ELISA, WB, ICC/IF

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

PP1 Catalytic Subunit, PPP1CA, APP0808-10 µg, APP0808-20 µg, APP0808-50 µg, APP0808-100 µg, APP0808-250 µg, APP0808-500 µg, APP0808-1 mg, APP0808-10, APP0808-20, APP0808-50, APP0808-100, APP0808-250, APP0808-500, APP0808-1

Cohen PT. (2002) J Cell Sci. 115 (2) :241-56.; ; Hsu LC, et al. (2006) Oncogene. 25 (40) :5517-26.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_002699

P62136

The cell lysate of HepG2 (45ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPP1CA (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

ICC/IF analysis of PPP1CA in HeLa cells. The cell was stained with APP0808 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .