Humaan PPAR gamma/NR1C3-antilichaam

PPAR gamma, also known as Peroxisome proliferator-activated receptor gamma, is member of the nuclear hormone receptor subfamily of transcription factors. It regulates transcription of genes involved in Insulin action, adipocyte differentiation, lipid metabolism and inflammation. It is implicated in numerous diseases including obesity, diabetes, atherosclerosis and cancer. It also activators include prostanoids, fatty acids, thiazolidinediones and N- (2-benzoylphenyl) tyrosine analogues. PPARG may modulate macrophage functions such as proinflammatory activities, and stimulate oxidized low-density lipoprotein (x-LDL) uptake.

Peroxisome proliferator-activated receptor gamma, NR1C3, CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARgamma, PPARG

Human

Human

Recombinant human PPARG (209-477aa) purified from E. coli.

AT4F5

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

PPAR g, ATGA0514-10 µg, ATGA0514-20 µg, ATGA0514-50 µg, ATGA0514-100 µg, ATGA0514-250 µg, ATGA0514-500 µg, ATGA0514-1 mg, ATGA0514-10, ATGA0514-20, ATGA0514-50, ATGA0514-100, ATGA0514-250, ATGA0514-500, ATGA0514-1

Berger J.. et al. (2002) Annu Rev Med . 53:409-35. ; ; Michalik L ., et al. (2006) Pharmacol Rev. 58 (4) :726-41.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Epigenomics (Transcription & Translation)

NP_619725

P37231

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPAR gamma antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate

Flow cytometry analysis of PPAR gamma in 293T cells. The cell was stained with ATGA0514 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

ICC/IF analysis of PPAR gamma in HeLa cells. The cell was stained with ATGA0514 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .