Humaan PPCS antilichaam

PPCS, also known as phosphopantothenate--cysteine ligase, catalyzes the first step in the biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5), which is an essential universal pathway in prokaryotes and eukaryotes. PPCS, one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine.

Phosphopantothenate--cysteine ligase, COAB, PPC synthetase

Human

Human

Recombinant human PPCS (1-311aa) purified from E. coli

AT10D11

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

PPCS, Phosphopantothenate--cysteine ligase, COAB, PPC synthetase, ATGA0443-10 µg, ATGA0443-20 µg, ATGA0443-50 µg, ATGA0443-100 µg, ATGA0443-250 µg, ATGA0443-500 µg, ATGA0443-1 mg, ATGA0443-10, ATGA0443-20, ATGA0443-50, ATGA0443-100, ATGA0443-250, ATGA0443-500, ATGA0443-1

Manoj N., et al. (2003) Structure. 11: 927-936. ; ; Christina Spry., et al. (2009) J Biol Chem. 284: 24904-24913.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_078940

Q9HAB8

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPCS antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Jurkat cell lysate ; ; Lane 2.: HepG2 cell lysate ; ; Lane 3.: HeLa cell lysate ; ; Lane 4.: A549 cell lysate ; ; Lane 5.: MCF7 cell lysate ; ; Lane 6.: SK-OV-3 cell lysate