Humaan PPME1-antilichaam

PPME1, also known protein phosphatase methylesterase-1 (PME1), catalyzes the demethylation and inactivation of protein phosphatase (PP2A), which is a multimeric phosphoserine/ threonine protein phosphatase associated with growth inhibition and cell cycle arrest. It can demethylate PP2A catalytic subunit in vitro and okadaic acid treatment is capable of inhibiting this reaction. It is conserved from yeast to human and contains a motif found in lipases having a catalytic triad activated serine as their active site nucleophile.

Protein phosphatase methylesterase 1, FLJ22226, PME-1

Human

Human

Recombinant human PPME1 (1-386aa) purified from E. coli

AT29G3

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

PPME1, Protein phosphatase methylesterase 1, FLJ22226, PME-1, ATGA0449-10 µg, ATGA0449-20 µg, ATGA0449-50 µg, ATGA0449-100 µg, ATGA0449-250 µg, ATGA0449-500 µg, ATGA0449-1 mg, ATGA0449-10, ATGA0449-20, ATGA0449-50, ATGA0449-100, ATGA0449-250, ATGA0449-500, ATGA0449-1

Ogris E., et al. (1999) J. Biol. Chem. 274: 14382-14391.; ; Gagnon S.N., et al. (2002) Biochem. J. 368: 263-271.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_057231

Q9Y570

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPME1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: K562 cell lysate ; ; Lane 3.: A549 cell lysate