Humaan PSMD11 antilichaam

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. PSMD11 is a member of the proteasome subunit S9 family that functions as a nonATPase subunit of the 19S regulator and is phosphorylated by AMP-activated protein kinase.

Proteasome 26S non-ATPase subunit 11, S9, Rpn6, p44.5, MGC3844

Human

Human

Recombinant human PSMD11 (1-422aa) purified from E. coli

AT2C7

Unconjugated

ELISA, WB, ICC/IF

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

PSMD11, ATGA0577-10 µg, ATGA0577-20 µg, ATGA0577-50 µg, ATGA0577-100 µg, ATGA0577-250 µg, ATGA0577-500 µg, ATGA0577-1 mg, ATGA0577-10, ATGA0577-20, ATGA0577-50, ATGA0577-100, ATGA0577-250, ATGA0577-500, ATGA0577-1

Ogris E., et al. (1999) J. Biol. Chem. 274: 14382-14391.; ; Gagnon S.N., et al. (2002) Biochem. J. 368: 263-271.

Fukuyama K, et al. Roasted Coffee Reduces β-Amyloid Production by Increasing Proteasomal β-Secretase Degradation in Human Neuroblastoma SH-SY5Y Cells. (Mol Nutr Food Res. 2018) {https://pubmed.ncbi.nlm.nih.gov/30144352/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Ubiquitination

NP_002806

O00231

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PSMD11 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: 293T cell lysate; ; Lane 3.: U87-MG cell lysate; ; Lane 4.: NIH-3T3 cell lysate;

ICC/IF analysis of PSMD11 in HeLa cells. The cell was stained with ATGA0577 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .