Humaan QPRT antilichaam

Quinolinate phosphoribosyltransferase, also known as QPRT, is a major enzyme in the catabolism of quinolinate. It is an intermediate in the tryptophannicotinamide adenine dinucleotide (NAD) pathway, leading to the production of nicotinic acid, carbon dioxide and pyrophosphate. Elevation of QPRT levels in the brain has been linked to the pathogenesis of neurodegenerative disorders such as epilepsy, Alzheimer's disease, and Huntington's disease.

Nicotinate nucleotide pyrophosphorylase (carboxylating), Nicotinate nucleotide pyrophosphorylase carboxylating, Quinolinate phosphoribosyltransferase, QPRTase, QAPRTase

Human

Human

Recombinant human QPRT (1-297aa) purified from E. coli

AT24C4

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

QPRT, ATGA0412-10 µg, ATGA0412-20 µg, ATGA0412-50 µg, ATGA0412-100 µg, ATGA0412-250 µg, ATGA0412-500 µg, ATGA0412-1 mg, ATGA0412-10, ATGA0412-20, ATGA0412-50, ATGA0412-100, ATGA0412-250, ATGA0412-500, ATGA0412-1

Cao H., et al. (2002) Biochemistry. 41 (10) : 3520-8.; ; Liu H., et al. (2007) J Mol Biol. 373 (3) : 755-63.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Neuroscience

NP_055113

Q15274

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human QPRT antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1 : HepG2 cell lysate; ; Lane 2 : K562 cell lysate

ICC/IF analysis of QPRT in Hep3B cells. The cell was stained with ATGA0412 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .