Humaan RalA/RalB antilichaam

RalA and RalB belong to subfamily of Ras-related GTPases. This protein mediates a distinct downstream signaling pathway from Ras that facilitates cellular transformation. It is also thought to be involved in numerous signaling cascades, including regulation of the cytoskeleton, vesicletrafficking, and endocytosis. RalA and RalB share 85% identity.

Ras-related protein Ral-A, RAL, Ras-related protein Ral-B

Human

Human

Recombinant human RALA (1-203aa) purified from E. coli

AT41E2

Unconjugated

ELISA, WB, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

RalB, Ras-related protein Ral-B, Ras related protein RalB, Ras related protein Ral B, RalA, Ras-related protein Ral-A, Ras related protein RalA, Ras related protein Ral A, ATGA0590-10 µg, ATGA0590-20 µg, ATGA0590-50 µg, ATGA0590-100 µg, ATGA0590-250 µg, ATGA0590-500 µg, ATGA0590-1 mg, ATGA0590-010, ATGA0590-020, ATGA0590-050, ATGA0590-100, ATGA0590-250, ATGA0590-500, ATGA0590-01M

Balasubramanian N., et al. (2009) Curr. Biol. 20:75-79.; ; Hunt MC., et al. (2005) J Lipid Res. 46, 2029-32.; ; Wildey G.M., et al. (1993), Biochem. Biophys. Res. Comm., 194: 552-559.; ; Jullien-Flores V., et al. (1995) . J. Biol. Chem. 270: 22473-22477.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Epigenetics and Nuclear Signaling

NP_005393

P11233

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RalA/RalB antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human RalA protein; ; Lane 2.: Recombinant human RalB protein; ; The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RalA/RalB antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: HepG2 cell lysate; ; Lane 3.: NIH3T3 cell lysate; ; Lane 4.: PC3 cell lysate; ; Lane 5.: U87MG cell lysate; ; {ATGA0590-WB2.jpg}

Flow cytometry analysis of RalA/RalB in HeLa cells. The cell was stained with ATGA0590 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .