Humaan RKIP/PEBP1 antilichaam

Phosphatidylethanolamine binding protein 1 (PEBP1), also known as Raf kinase inhibitor protein (RKIP), is a member of the phosphatidylethanolamine-binding protein family and a serine protease inhibitor which inhibits thrombin, neuropsin. PEBP1 plays a pivotal modulatory role in several protein kinase signaling cascades. Protein kinase C (PKC) phosphorylates PEBP1, resulting in release of Raf-1 and activation of MEK and ERK. Expressed in many tissues, PEBP1 is implicated in the regulation of such physiological processes as membrane biosynthesis, spermatogenesis, neural development, and metastasis suppression.

Phosphatidylethanolamine binding protein 1, PBP, Prostatic binding protein, RKIP, HCNP, HCNPpp, PEBP, Raf kinase inhibitory protein, Hippocampal cholinergic neurostimulating peptide

Human

Human

Recombinant human PEBP1 (1-187aa) purified from E. coli

AT4B11

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

RKIP, PEBP1, ATGA0456-10 µg, ATGA0456-20 µg, ATGA0456-50 µg, ATGA0456-100 µg, ATGA0456-250 µg, ATGA0456-500 µg, ATGA0456-1 mg, ATGA0456-10, ATGA0456-20, ATGA0456-50, ATGA0456-100, ATGA0456-250, ATGA0456-500, ATGA0456-1

Keller ET., et al. (2004) Biochem Pharmacol. 68 (6) : 1049-53. ; ; Park S., et al. (2005) Oncogene. 24 (21) : 3535-40.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cell Cycle

NP_002558

P30086

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PEBP1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1 : HeLa cell lysate; ; Lane 2 : SKOV3 cell lysate; ; Lane 3 : A549 cell lysate; ; Lane 4 : HepG2 cell lysate; ; Lane 5 : MCF7 cell lysate; ; Lane 6 : K562 cell lysate; ; Lane 7 : LnCap cell lysate; ; Lane 8 : Ramos cell lysate

ICC/IF analysis of PEBP1 in HeLa cells line, monoclonal anti-human PEBP1 antibody (1:100) stained with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .