Humaan S100A11 antilichaam

S100A11 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein may function in motility, invasion, and tubulin polymerization.

S100 calcium binding protein A11, MLN70, S100C

Human

Human

Recombinant human S100A11 (1-105aa) purified from E. coli

AT20D11

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

S100A11, S100 calcium binding protein A11, MLN70, S100C, ATGA0434-10 µg, ATGA0434-20 µg, ATGA0434-50 µg, ATGA0434-100 µg, ATGA0434-250 µg, ATGA0434-500 µg, ATGA0434-1 mg, ATGA0434-10, ATGA0434-20, ATGA0434-50, ATGA0434-100, ATGA0434-250, ATGA0434-500, ATGA0434-1

Sakaguchi M., et al. (2003) J Cell Biol. 163: 825-35.; ; Settmacher U., et al. (2005) Int. J. Oncol. 28: 195-200.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cell Cycle

NP_005611

P31949

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human S100A11 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : HeLa cell lysate ; ; Lane 2. : MCF7 cell lysate ; ; Lane 3. : PC3 cell lysate ; ; Lane 4. : THP1 cell lysate ; ; Lane 5. : U87MG cell lysate ; ; Lane 6. : A427 cell lysate ; ; Lane 7. : SK-OV-3 cell lysate ; ; Lane 8. : A549 cell lysate

Flow cytometry analysis of S100a11 in HeLa cells. The cell was stained with ATGA0434 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) . Flow cytometry analysis of S100a11 in A549 cells. The cell was stained with ATGA0434 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) . {2004010373111810.jpg}

ICC/IF analysis of S100A11 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human S100A11 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .