Humaan Serpin B5/Maspin antilichaam

Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 5, also known as Maspin, is a tumor suppressor protein of serine proteinase inhibitor family. It plays an essential role in embryonic development through critical roles in cell adhesion. Also, it is present in normal breast and prostatic epithelial cells but downregulated in the respective carcinomas.

Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 5, Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 5, Serpin B5, Protease inhibitor 5, SERPINB5, PI5, Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 5 Serine (or cysteine) peptidase inhibitor, clade B, member 5, Ovalbumin, PI-5, Serpin peptidase inhibitor, Serpin peptidase inhibitor, clade B (ovalbumin), member 5, SERPINB5, Spi5, Spi7

Human

Human

Recombinant Maspin (1-375aa) purified from E. coli

AT2N6

Unconjugated

ELISA, WB, ICC/IF

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

Serpin B5, Maspin, ATGA0167-10 µg, ATGA0167-20 µg, ATGA0167-50 µg, ATGA0167-100 µg, ATGA0167-250 µg, ATGA0167-500 µg, ATGA0167-1 mg, ATGA0167-10, ATGA0167-20, ATGA0167-50, ATGA0167-100, ATGA0167-250, ATGA0167-500, ATGA0167-1

Lockett J. et al., (2006) J Cell Biochem 97 (4) :651-660.; ; Khalkhali Ellis Z. et al., (2006) Clin Cancer Res 12 (24) :7279-7283.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Development

NP_002630

P36952

The Cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Serpin B5/Maspin antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate

Immunofluorescence of human HeLa cells stained with PI (Red) and monoclonal anti-Maspin antibody (1:500) with Alexa 488 (Green) .