Humaan SHMT1-antilichaam

Serine hydroxymethyltransferase 1, also known as SHMT1, belongs to the SHMT family. This protein is cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5 10-methylene tetrahydrofolate. Also, SHMT1 preferentially supplies one-carbon units for thymidylate biosynthesis, depletes methylenetetrahydrofolate pools for S-adenosylmethionine (SAM) synthesis by synthesizing serine, sequesters 5-methyltetrahydrofolate and inhibits.

Serine hydroxymethyltransferase 1, CSHMT, SHMT

Human

Human

Recombinant human SHMT1 (1-483aa) purified from E. coli

AT26E5

Unconjugated

ELISA, WB, ICC/IF

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

SHMT1, ATGA0413-10 µg, ATGA0413-20 µg, ATGA0413-50 µg, ATGA0413-100 µg, ATGA0413-250 µg, ATGA0413-500 µg, ATGA0413-1 mg, ATGA0413-10, ATGA0413-20, ATGA0413-50, ATGA0413-100, ATGA0413-250, ATGA0413-500, ATGA0413-1

Garrow TA., et al. (1993) J Biol Chem. 268 (16) : 11910-6.; ; Perry C., et al. (2005) J Biol Chem. 280 (1) : 396-400. ; ; Pinthong C., et al. (2014) FEBS J. 281 (11) : 2570-83.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Metabolism

AAH07979

P34896

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SHMT1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: A549 cell lysate ; ; Lane 2.: HeLa cell lysate; ; Lane 3.: NIH-3T3 cell lysate ; ; Lane 4.: LnCaP cell lysate

ICC/IF analysis of SHMT1 in Hep3B cells. The cell was stained with ATGA0413 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .