Humaan slak antilichaam

SNAI1, also known as Snail homolog 1, is involved in the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. This protein binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. Both SNAI1 and SLUG belong to the snail C2H2-type zinc-finger protein family. SLUG is a transcriptional repressor, involved in the generation and migration of neural crest cells. SNAI1 is expressed in mesenchymal, epithelial cell lines and highest expression in kidney.

Snail homolog 1, dJ710H13.1, SLuGH2, SNA, SNAH, SNAIL, SNAIL1

Human

Human

Recombinant human SNAI1 (1-264aa) purified from E. coli

AT2D5

Unconjugated

ELISA, WB

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

Snail, SNAI1, ATGA0288-10 µg, ATGA0288-20 µg, ATGA0288-50 µg, ATGA0288-100 µg, ATGA0288-250 µg, ATGA0288-500 µg, ATGA0288-1 mg, ATGA0288-10, ATGA0288-20, ATGA0288-50, ATGA0288-100, ATGA0288-250, ATGA0288-500, ATGA0288-1

Batlle. E., et al. (2000) Nat Cell Biol 2: 84-89.; ; Yook. J.I., et al. (2005) J Biol Chem 280: 11740-11748.; ; Twigg. S.R. and Wilkie. A.O.M. (1999) Hum Genet 105: 320-326.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Epigenomics (Transcription & Translation)

NP_005976

O95863

The cell lysate of A549 (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SNAI1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.