Humaan SMAC/Diablo antilichaam

SMAC/Diablo is one of the proapoptotic proteins. SMAC promotes caspase activation in the cytochrome c/Apaf-1/caspase-9 pathway by binding to inhibitor of apoptosis proteins (IAPs) and removing their inhibitory activity. In healthy cells, SMAC is a mitochondrial protein, but when cells undergo apoptosis, SMAC is released into the cytosol. SMAC is ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes

Diablo isoform 1, SMAC3, DIABLO-S, FLJ10537, FLJ25049, Diablo isoform 1, SMAC/Diablo, Diablo isoform 1 0610041G12Rik, DBOH, Diablo homolog, DIABLO S, Diablo homolog (Drosophila), Mitochondrial Smac protein, Diablo homolog mitochondrial, SMAC 3, Smac protein, Direct IAP binding protein with low pI, Second mitochondria derived activator of caspase

Human

Human

Recombinant human SMAC/Diablo (56-239aa) purified from E. coli

AT19F2

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

SMADiablo, ATGA0266-10 µg, ATGA0266-20 µg, ATGA0266-50 µg, ATGA0266-100 µg, ATGA0266-250 µg, ATGA0266-500 µg, ATGA0266-1 mg, ATGA0266-10, ATGA0266-20, ATGA0266-50, ATGA0266-100, ATGA0266-250, ATGA0266-500, ATGA0266-1

McNeish. I.A., et al. (2003) Exp Cell Res 286 (2) : 186-198.; ; Yu. J., et al. (2007) Oncogene 26 (29) : 4189-4198.; ; Vucic. D., et al. (2002) J Biol Chem 277 (14) : 12275-12279.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Cancer

NP_063940

Q9NR28

The cell lysate of Jurkat (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SMAC antibody (1:3000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SMAC antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: A431 cell lysate; ; Lane 3.: Hep3B cell lysate; ; Lane 4.: MCF7 cell lysate {ATGA0266-WB2.jpg}

Flow cytometry analysis of SMAC in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of SMAC in HeLa cells. The cell was stained with ATGA0266 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .