Humaan SNAP25 antilichaam

The synaptosomal-associated protein (SNAP25) is an essential component of the core complex that mediates presynaptic vesicle trafficking. Thus, SNAP25 is directly involved in the release of neurotransmitters.

Synaptosomal-associated protein 25 isoform SNAP25B, SNAP25, SNAP, SNAP25, Synaptosomal-associated protein, His- tag, SNAP-25, Synaptosomal-associated 25 kDa protein, Super protein, SuP, Synaptosomal-associated protein 25, Synaptosomal-associated protein 25 isoform SNAP25B bA416N4.2, Bdr, dJ1068F16.2, FLJ23079, HGNC:11132, MGC105414, MGC139754, Resistance to inhibitors of cholinesterase 4 homolog, RIC 4, RIC4, SEC9, SNAP 25, SNAP-25B, SNP 25, sp, Synaptosomal associated 25 kDa protein, Synaptosomal associated protein, Synaptosomal Associated Protein 25, Synaptosomal associated protein 25kDa

Human

Human

Recombinant human SNAP25 (1-206aa) purified from E. coli

400000000000

Unconjugated

ELISA, WB, ICC/IF

By protein-G affinity chromatography

1 mg/mL (determined by BCA assay)

SNAP25, ASN0720-10 µg, ASN0720-20 µg, ASN0720-50 µg, ASN0720-100 µg, ASN0720-250 µg, ASN0720-500 µg, ASN0720-1 mg, ASN0720-10, ASN0720-20, ASN0720-50, ASN0720-100, ASN0720-250, ASN0720-500, ASN0720-1

Schulz JR et al., (1998) J Biol Chem 273 (38) :24355-9.; ; Tafoya LC et al., (2006) J Neurosci 26 (30) :7826-38.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Neuroscience

NP_570824

P60880

The tissue lysate (30ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SNAP25 antibody (1:2000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse brain tissue lysate The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SNAP25 antibody (1:3000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: SNAP25 Transfected 293T cell lysate {2004010049531236.jpg}

ICC/IF analysis of SNAP25 in U87MG cells. The cell was stained with ASN0720 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .