Humaan SOD1/Cu-Zn SOD antilichaam

Cu/Zn superoxide dismutase (SOD1), is a major antioxidant enzyme that catalyzes the conversion of superoxide anion to hydrogen peroxide and molecular oxygen. SOD1 facilitates the dismutation of oxygen radicals to hydrogen peroxide and also catalyzes pro-oxidant reactions, which include the peroxidase activity and hydroxyl radical generating activity. Also over 100 distinct SOD1 inherited mutations have been identified in the familial form of amyotrophic lateral sclerosis (ALS), a progressive degenerative disease of motor neurons. The mechanism by which mutant SOD1 induces the neurodegeneration observed in ALS is still unclear.

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Human

Human

Recombinant human SOD1 (1-154aa) purified from E. coli

AT6F1

Unconjugated

ELISA, WB, ICC/IF, FACS

By protein-A affinity chromatography

1 mg/mL (determined by BCA assay)

SOD1, Cu-Zn SOD, ATGA0405-10 µg, ATGA0405-20 µg, ATGA0405-50 µg, ATGA0405-100 µg, ATGA0405-250 µg, ATGA0405-500 µg, ATGA0405-1 mg, ATGA0405-10, ATGA0405-20, ATGA0405-50, ATGA0405-100, ATGA0405-250, ATGA0405-500, ATGA0405-1

Deng HX., et al. (1993) Science. 261 (5124) : 1047-51.; ; Valentine JS., et al. (2003) Proc Natl Acad Sci USA. 100 (7) : 3617-22.; ; Rosen DR., et al. (1993) Nature. 362 (6415) : 59-62.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Signal Transduction

NP_000445

P00441

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SOD1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : A549 cell lysate; ; Lane 2. : A431 cell lysate; ; Lane 3. : 293T cell lysate; ; Lane 4. : PC3 cell lysate; ; Lane 5. : U87MG cell lysate; ; The recombinant proteins (20ng) of SOD two isoform were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SOD1 (1:1000) .; ; Lane 1 : Recombinant protein SOD1; ; Lane 2 : Recombinant protein SOD2 ; ; {ATGA0405-WB2.jpg}

Flow cytometry analysis of SOD1 in Hep3B cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

ICC/IF analysis of SOD1 in Hep3B cells. The cell was stained with ATGA0405 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .